Goldfish brain (Na+,K+)-ATPase: purification of the catalytic polypeptide and production of specific antibodies.

The denatured catalytic polypeptide of (Na+,K+)-ATPase of goldfish brain was purified and identified as the 32P-labeled phosphoprotein. The protein served as immunogen for the preparation of rabbit antisera for immunohistochemical application t o goldfish tissue sections, using t h e peroxidase-antiperoxidase indirect method. Labeling in brain cross-sections appears primarily in fibers of the optic nerve layer of the tectum. In optic nerve cross sections, labeling is restricted to fiber bundles.